The LyoStar II features an enhanced cascade refrigeration system that has more horsepower to provide ample cooling to -70°C. The use of RTD’s for temperature measurement ensures shelf temperature stability and accuracy of ±0.5°C. Additional mechanical enhancements enable the LyoStar II to cool at rates in excess of 2.5°C/minute. This allows more flexibility in developing cycles for scale-up. The shelf to condenser surface ratio ensures ample lyophilization efficiency for even the most aggressive cycles. Enhancements to the human-machine interface create a simplified intuitive operating system that requires minimal training. A new touch screen computer facilitates data entry and cycle monitoring.